How is the KasII Hairpin Antisense work? |
Written by Tam Nguyen |
Sunday, 22 February 2009 16:46 |
Gas chromatograph-mass spectrometer (GCMS) analysis fatty acid single seed of KasII-Hairpin Antisense seed Suppression of kasII enzyme activity by KasII Hairpin Antisense due to reducing 18:0, 18:1 (delta 9), 18:2 and 18:3 fatty acid (diagram at very top) and result analysis fatty acid by GCMS of transgenic (bottom) and control (top) seeds showed that an accumulation of 16:0 Fatty acid which due to enhance a little bit of 16:1 delta 9 and 18:1 delta11 fatty acids (Source: unpublished)
Schematic diagram showing the relationship of DNA elements comprising antisense (AS), hairpin (HP) and hairpin antisense (HPAS) constructs (Source: Journal of the American Oil Chemists' Society) A schematic representation of the possible roles of hairpin and antisense in suppressing a target gene (Source: Journal of the American Oil Chemists' Society) |
Last Updated on Saturday, 28 February 2009 15:36 |